Relative quantification of deuterated omega-3 and -6 fatty acids and their lipid turnover in PC12 cell membranes using TOF-SIMS
Artikel i vetenskaplig tidskrift, 2018

Understanding FA metabolism and lipid synthesis requires a lot of information about which FAs and lipids are formed within the cells. We focused on the use of deuterated substrates of 100 μM α-linolenic acid and linoleic acid to determine the relative amounts of their converted PUFAs and specific phospholipids that are incorporated into cell plasma membranes. Time-of-flight secondary ion mass spectrometry (TOF-SIMS) was used to image and analyze lipids in model cell membranes with and without FA treatment. Because of its high spatial resolution, TOF-SIMS can be used to simultaneously provide both chemical information and distribution of various molecules in the sample surface down to the subcellular scale. Data obtained from this analysis of isotopes in the cell samples were used to calculate the relative amounts of long-chain PUFAs and phospholipids from their precursors, α-linolenic acid and linoleic acid. Our results show that the FA treatments induced an increase in the amounts of α-linolenic acid and linoleic acid and their long-chain conversion products. Moreover, an enhanced level of phospholipid turnover of these FAs in lipids such as phosphatidylcholines, phosphatidylethanolamines, and phosphatidylinositols was also observed in the cell plasma membrane.

linoleic acid

lipid turnover

time-of-flight secondary ion mass spectrometry

α-linolenic acid

phospholipids

fatty acids

Författare

Thuy Mai Hoang Philipsen

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Göteborgs universitet

Sanna Sämfors

Göteborgs universitet

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Per Malmberg

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Göteborgs universitet

Andrew Ewing

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Göteborgs universitet

Journal of Lipid Research

0022-2275 (ISSN) 1539-7262 (eISSN)

Vol. 59 11 2098-2107

Ämneskategorier

Annan medicinsk grundvetenskap

Biofysik

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

DOI

10.1194/jlr.M087734

PubMed

30206182

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Senast uppdaterat

2022-10-23