Food-induced changes of lipids in rat neuronal tissue visualized by ToF-SIMS imaging
Artikel i vetenskaplig tidskrift, 2016

Time of flight secondary ion mass spectrometry (ToF-SIMS) was used to image the lipid localization in brain tissue sections from rats fed specially processed cereals (SPC). An IonTof 5 instrument equipped with a Bi cluster ion gun was used to analyze the tissue sections. Data from 15 brain samples from control and cereal-fed rats were recorded and exported to principal components analysis (PCA). The data clearly show changes of certain lipids in the brain following cereal feeding. PCA score plots show a good separation in lipid distribution between the control and the SPC-fed group. The loadings plot reveal that the groups separated mainly due to changes in cholesterol, vitamin E and c18:2, c16:0 fatty acid distribution as well as some short chain monocarboxylic fatty acid compositions. These insights relate to the working mechanism of SPC as a dietary supplement. SPC is thought to activate antisecretory factor (AF), an endogenous protein with regulatory function for inflammation and fluid secretion. These data provide insights into lipid content in brain following SPC feeding and suggest a relation to activating AF.

menieres-disease

brain-tissue

cell-membranes

cholesterol

protein

Science & Technology - Other Topics

alzheimers-disease

ion mass-spectrometry

localization

phosphatidylethanolamine

antisecretory factor

Författare

[Person ab4b5fa0-c17b-4d1f-9d01-aa85f0b8493e not found]

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

[Person 9b6c309b-066b-46b7-a661-6726cfd58f46 not found]

Göteborgs universitet

[Person 529c2cd3-5a0e-4b0b-ae2b-e898f7ad6d58 not found]

Göteborgs universitet

[Person 043d5c25-1319-44f5-b3d8-6d0372cd79fb not found]

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Göteborgs universitet

[Person ee913871-6062-4ebf-b494-1dff9dc9f9de not found]

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Scientific Reports

2045-2322 (ISSN)

Vol. 6 32797

Ämneskategorier

Biologiska vetenskaper

Kemi

DOI

10.1038/srep32797

Mer information

Skapat

2017-10-08