Quantitative Measurement of Transmitters in Individual Vesicles in the Cytoplasm of Single Cells with Nanotip Electrodes
Artikel i vetenskaplig tidskrift, 2015

The quantification of vesicular transmitter content is important for studying the mechanisms of neurotransmission and malfunction in disease, and yet it is incredibly difficult to measure the tiny amounts of neurotransmitters in the attoliter volume of a single vesicle, especially in the cell environment. We introduce a novel method, intracellular vesicle electrochemical cytometry. A nanotip conical carbon-fiber microelectrode was used to electrochemically measure the total content of electroactive neurotransmitters in individual nanoscale vesicles in single PC12 cells as these vesicles lysed on the electrode inside the living cell. The results demonstrate that only a fraction of the quantal neurotransmitter content is released during exocytosis. These data support the intriguing hypothesis that the vesicle does not open all the way during the normal exocytosis process, thus resulting in incomplete expulsion of the vesicular contents.

Carbon fibers

Carbon

Conical carbon

Neurophysiology

Quantitative measurement

catecholamines

Electrodes

Nanotips

vesicles

exocytosis

electrochemistry

Nano-electrodes

Intracellular vesicles

Microelectrodes

Electroactive neurotransmitters

nanoelectrodes

Transmitters

Författare

Li Xianchan

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Göteborgs universitet

Soodabeh Majdi

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Johan Dunevall

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Hoda Mashadi Fathali

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Andrew Ewing

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Göteborgs universitet

Angewandte Chemie - International Edition

1433-7851 (ISSN) 1521-3773 (eISSN)

Vol. 54 11978-11982

Ämneskategorier

Analytisk kemi

DOI

10.1002/anie.201504839

PubMed

26266819