Spatial resolution of exocytosis across a single cell by a microwell-based individually addressable thin film ultra-microelectrode array
Paper i proceeding, 2013

We report the fabrication and characterization of micro well-based individually addressable ultra-microelectrode arrays (MEAs) and their application to spatially and temporally resolved detection of neurotransmitter release across a single pheochromocytoma (PC 12) cell. The MEAs consist of sixteen 4-μm-width square microelectrodes, or twenty-five 3-μm-width square microelectrodes, or thirty-six 2-jim-width square microelectrodes. Each MEA is tightly defined in a 30×30 μm square area, which is further encased inside a 40×40 μm SU-8 microwell. We demonstrate the excellent stability and reproducibility of these microelectrodes by using cyclic voltammetry and we have performed recording of spatially resolution of single cell exocytosis with multiple ultra-microelectrodes in 2-μm resolution.

Microwell-based ultra-microelectrode array

Spatial and temporal resolution

Single cell exocytosis


Jun Wang

Göteborgs universitet

Chalmers, Kemi- och bioteknik, Analytisk kemi

R. Trouillon

Göteborgs universitet

Johan Dunevall

Chalmers, Kemi- och bioteknik, Analytisk kemi

Andrew Ewing

Chalmers, Kemi- och bioteknik, Analytisk kemi

Göteborgs universitet

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013

Vol. 1 392-394