Spatial resolution of exocytosis across a single cell by a microwell-based individually addressable thin film ultra-microelectrode array
Paper in proceedings, 2013

We report the fabrication and characterization of micro well-based individually addressable ultra-microelectrode arrays (MEAs) and their application to spatially and temporally resolved detection of neurotransmitter release across a single pheochromocytoma (PC 12) cell. The MEAs consist of sixteen 4-μm-width square microelectrodes, or twenty-five 3-μm-width square microelectrodes, or thirty-six 2-jim-width square microelectrodes. Each MEA is tightly defined in a 30×30 μm square area, which is further encased inside a 40×40 μm SU-8 microwell. We demonstrate the excellent stability and reproducibility of these microelectrodes by using cyclic voltammetry and we have performed recording of spatially resolution of single cell exocytosis with multiple ultra-microelectrodes in 2-μm resolution.

Microwell-based ultra-microelectrode array

Spatial and temporal resolution

Single cell exocytosis


Jun Wang

University of Gothenburg

Chalmers, Chemical and Biological Engineering, Analytical Chemistry

R. Trouillon

University of Gothenburg

Johan Dunevall

Chalmers, Chemical and Biological Engineering, Analytical Chemistry

Andrew Ewing

Chalmers, Chemical and Biological Engineering, Analytical Chemistry

University of Gothenburg

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013

Vol. 1 392-394

Subject Categories

Chemical Engineering



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