Zinc Regulates Chemical-Transmitter Storage in Nanometer Vesicles and Exocytosis Dynamics as Measured by Amperometry
Artikel i vetenskaplig tidskrift, 2017

We applied electrochemical techniques with nano-tip electrodes to show that micromolar concentrations of zinc not only trigger changes in the dynamics of exocytosis, but also vesicle content in a model cell line. The vesicle catecholamine content in PC12 cells is significantly decreased after 100 mm zinc treatment, but, catecholamine release during exocytosis remains nearly the same. This contrasts with the number of molecules stored in the exocytosis vesicles, which decreases, and we find that the amount of catecholamine released from zinc-treated cells reaches nearly 100% content expelled. Further investigation shows that zinc slows down exocytotic release. Our results provide the missing link between zinc and the regulation of neurotransmitter release processes, which might be important in memory formation and storage.

Chromaffin

vesicle content

Extended Kiss

exocytosis

Protein-Kinase-C

PC12 Cells

amperometry

Release

Fusion Pore

Cells

Quantal Size

zinc

Kiss-and-Run

In-Vitro

Actin

cytometry

Författare

Lin Ren

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Masoumeh Dowlatshahi Pour

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Soodabeh Majdi

Göteborgs universitet

Li Xianchan

Göteborgs universitet

Per Malmberg

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Andrew Ewing

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Angewandte Chemie - International Edition

1433-7851 (ISSN) 1521-3773 (eISSN)

Vol. 56 18 4970-4975

Ämneskategorier

Oorganisk kemi

DOI

10.1002/anie.201700095