Zinc Regulates Chemical-Transmitter Storage in Nanometer Vesicles and Exocytosis Dynamics as Measured by Amperometry
Journal article, 2017
We applied electrochemical techniques with nano-tip electrodes to show that micromolar concentrations of zinc not only trigger changes in the dynamics of exocytosis, but also vesicle content in a model cell line. The vesicle catecholamine content in PC12 cells is significantly decreased after 100 mm zinc treatment, but, catecholamine release during exocytosis remains nearly the same. This contrasts with the number of molecules stored in the exocytosis vesicles, which decreases, and we find that the amount of catecholamine released from zinc-treated cells reaches nearly 100% content expelled. Further investigation shows that zinc slows down exocytotic release. Our results provide the missing link between zinc and the regulation of neurotransmitter release processes, which might be important in memory formation and storage.
Chromaffin
vesicle content
Extended Kiss
exocytosis
Protein-Kinase-C
PC12 Cells
amperometry
Release
Fusion Pore
Cells
Quantal Size
zinc
Kiss-and-Run
In-Vitro
Actin
cytometry
Author
Lin Ren
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Masoumeh Dowlatshahi Pour
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Soodabeh Majdi
University of Gothenburg
Li Xianchan
University of Gothenburg
Per Malmberg
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Andrew Ewing
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Angewandte Chemie - International Edition
1433-7851 (ISSN) 1521-3773 (eISSN)
Vol. 56 18 4970-4975Subject Categories
Inorganic Chemistry
DOI
10.1002/anie.201700095