Per Malmberg
My research is firmly rooted in analytical chemistry and especially mass spectrometry, and I have been working with and developing secondary ion mass spectrometry (SIMS) based methodologies, such as ToF-SIMS and NanoSIMS, but also matrix assisted laser desorption ionization (MALDI) methods for mass spectrometry imaging (MSI). In MSI you render chemical maps of an analyte by acquiring mass spectra information from individual points in a sample thereby creating a spatially resolved image with elemental and/or molecular information. Mainly using these techniques my ongoing research has three major focus areas (1) Metals in biology (2) Drug development and drug safety (3) Surfaces and interfaces. The focus areas are described briefly below.
Metals in biology
I’m very passionate about understanding the role and function of metals in biology. In the Swedish Research Council funded project (PI Per Malmberg) “Molecular imaging at the synaptic level: the role of synaptic zinc in traumatic brain injury and neurodegenerative disease” I started to develop methods for tracing primarily zinc, but now also other metals in the brain and the nervous system. The storage and release of metals in cells is involved in several diseases and coupled to increased oxidative stress in the cells. Recently, I have come to focus not only on the central nervous system but also the endocrine system and the gut since the handling of metals in cells is more complex and has wider implications than I initially grasped.
My metals in biology approach has led to several interesting publications and I had a shared PhD student that graduated in this topic in February 2018. Here we have also studied subcellular localization of zinc, iron and copper using nanoscale secondary ion mass spectrometry (NanoSIMS). Using this approach, we have demonstrated sub 25 nm NanoSIMS imaging of iron on cells. Recently we also published data on iron in Chronic Obstructive Pulmonary Disease (COPD) (co-funded by AstraZeneca) as well as metal allergens in skin tissue.
The role of MSI for drug development and drug safety assessment
Another focus is on methods for drug development and drug safety assessment in the Innovative Medicine Initiative (IMI/EU) funded project TRISTAN (WP4) where we trace drugs and metabolites with mass spectrometry and aim to develop new methods to probe drug safety. Here I have studied how 89Zr-Trastuzumab, a metal tagged monoclonal antibody used to treat breast cancer interacts with tissue and cells and the HER2 receptor. This bridges into metals in biology and we focus on metal-chelates that can be analyzed with both conventional PET as well as MSI techniques. Being able to localize metal-chelates with high precision, i.e on a cellular or sub-cellular scale, is of great importance and will lead to a better understanding of drug interaction, in especially cancer treatment.
Drug development is the focus of a collaborative project together with AstraZeneca. Here we strive to develop techniques for sub-cellular drug/drug-target localization and quantification together with CAMECA.
Surfaces and interfaces
The surfaces of cells and materials is another interest of mine and I have been exploring this in several ways ranging from studies on how graphene interacts with surfaces to how hydrogen/deuterium in integrated in oxides. I’ve had an especially fruitful collaboration with the high temperature corrosion center (HTC) at Chalmers, where we have done some pioneering work analyzing oxide scale build-up using 18O and deuterium labeling. Another interest of mine is biomaterials and the interaction of materials, such as titanium and magnesium, with cells and tissue, where I have published a number of papers over the years. Mapping the initial reaction of implants with blood is important for the understanding of the healing process in bone. It is similarly important to map what happens in the soft tissue itself by the implant contact and to understand the corrosion processes ongoing on the surface of the implant. Here MSI has provided a more complete picture than many other approaches can. This by being able to map both metals and the organic components simultaneously.
Showing 107 publications
Field Manipulation of Band Properties in Infrared Spectra of Thin Films
Evidence for intracellular silicon uptake in RAW264.7 cells, possibly mediated by aquaporin 9
Distinct Cholesterol Localization in Glioblastoma Multiforme Revealed by Mass Spectrometry Imaging
Exploiting Mass Spectrometry to Unlock the Mechanism of Nanoparticle-Induced Inflammasome Activation
Skin permeation studies of chromium species – Evaluation of a reconstructed human epidermis model
Bonding between π-Conjugated Polycations and Monolayer Graphene: Decisive Role of Anions
Correlative High-Resolution Imaging of Iron Uptake in Lung Macrophages
Topical Application of Metal Allergens Induces Changes to Lipid Composition of Human Skin
On the function of lead (Pb) in machining brass alloys
Animal- free skin permeation analysis using mass spectrometry imaging
Mapping the chemistry of hair strands by mass spectrometry imaging - A Review
Evidence for Electron Transfer between Graphene and Non-Covalently Bound pi-Systems
Multiple growth of graphene from a pre-dissolved carbon source
Copper nanoparticles have negligible direct antibacterial impact
Subcellular Mass Spectrometry Imaging and Absolute Quantitative Analysis across Organelles
ToF-SIMS imaging of dual biomolecular monolayer gradients
Molecular insights into hypomineralized enamel
Contact allergy to beeswax and propolis among patients with cheilitis or facial dermatitis
Mass spectrometry imaging as a novel approach to measure hippocampal zinc
Interplay of water and reactive elements in oxidation of alumina-forming alloys
Soft-tissue evidence for homeothermy and crypsis in a Jurassic ichthyosaur
Graphene Oxide Elicits Membrane Lipid Changes and Neutrophil Extracellular Trap Formation
Nano Secondary Ion Mass Spectrometry Imaging of Dopamine Distribution Across Nanometer Vesicles
Formation of hydroxyapatite on titanium implants in vivo precedes bone-formation during healing
Mg-corrosion, hydroxyapatite, and bone healing
Antisecretory Factor (AF) egg-yolk peptides reflects the intake of AF-activating feed in hens
Multimodal Imaging of Chemically Fixed Cells in Preparation for NanoSIMS
Mineralization at Titanium Surfaces is a Two-Step Process.
Food-induced changes of lipids in rat neuronal tissue visualized by ToF-SIMS imaging
A ToF-SIMS investigation of the corrosion behavior of Mg alloy AM50 in atmospheric environments
XRMA and ToF-SIMS Analysis of Normal and Hypomineralized Enamel.
Spatial neuroproteomics using imaging mass spectrometry
Analysis of liposome model systems by time-of-flight secondary ion mass spectrometry
Increase of Compact Bone Thickness in Rat Tibia after Implanting MgO into the Bone Marrow Cavity
Using imaging ToF-SIMS data to determine the cell wall thickness of fibers in wood
Analysis of As- and Hg-Species in Metal-Resistant Oral Bacteria, by Imaging ToF-SIMS
High-resolution, imaging TOF-SIMS: novel applications in medical research
High-resolution imaging and proteomics of peptide fragments by TOF-SIMS
Molecular imaging of lipids in cells and tissues
Bioimaging TOF-SIMS: High resolution 3D imaging of single cells.
Localization of lipids in the aortic wall with imaging TOF-SIMS
Localization of cholesterol in rat cerebellum with imaging TOF-SIMS Effect of tissue preparation
High resolution imaging by organic secondary ion mass spectrometry.
Depth profiling of cells and tissues by using C-60(+) and SF5+ as sputter ions
Imaging of lipids in human adipose tissue by cluster ion TOF-SIMS
Localization of Na+ and K+ in rat cerebellum with imaging TOF-SIMS
Distribution of cholesterol and galactosylceramide in rat cerebellar white matter.
Imaging TOF-SIMS of rat kidney prepared by high-pressure freezing.
Development of a wound dressing targeting neutrophil function.
Bioimaging TOF-SIMS of tissues by gold ion bombardment of a silver-coated thin section.
Bioimaging TOF-SIMS: localization of cholesterol in rat kidney sections.
Subcellular localisation of cholesterol and phosphocholine with pattern-recognition-imaging-TOF-SIMS
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Showing 6 research projects
Dermal uptake of polycyclic aromatic compounds by work environment exposure
Kemisk avbildning för hudpermeationsstudier - ett metod för att ersätta djurförsök
Chemical imaging analysis of skin permeation of sensitizing and toxic metals
Chemical imaging for skin permeation studies – an analytical approach to replace animal experiments