Altered Lipid Composition of Secretory Cells Following Exposure to Zinc Can Be Correlated to Changes in Exocytosis
Journal article, 2019

A micromolar concentration of zinc has been shown to significantly change the dynamics of exocytosis as well as the vesicle contents in a model cell line, providing direct evidence that zinc regulates neurotransmitter release. To provide insight into how zinc modulates these exocytotic processes, neurotransmitter release and vesicle content were compared with single cell amperometry and intracellular impact vesicle cytometry with a range of zinc concentrations. Additionally, time-of-flight secondary ion mass spectrometry (ToF-SIMS) images of lipid distributions in the cell membrane after zinc treatment correlate to changes in exocytosis. By combining electrochemical techniques and mass spectrometry imaging, we proposed a mechanism by which zinc changes the fusion pore and the rate of neurotransmitter release by changing lipid distributions and results in the modulation of synaptic strength and plasticity.

regulated exocytosis

lipid compostition

zinc

vesicle content

amperometry

Author

Lin Ren

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Masoumeh Dowlatshahi Pour

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Per Malmberg

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Andrew Ewing

University of Gothenburg

Chemistry - A European Journal

0947-6539 (ISSN) 1521-3765 (eISSN)

Vol. 25 21 5406-5411

Subject Categories

Food Engineering

Other Chemistry Topics

Biophysics

Infrastructure

Chemical Imaging Infrastructure

DOI

10.1002/chem.201900010

More information

Latest update

5/14/2019