Altered Lipid Composition of Secretory Cells Following Exposure to Zinc Can Be Correlated to Changes in Exocytosis
Artikel i vetenskaplig tidskrift, 2019

A micromolar concentration of zinc has been shown to significantly change the dynamics of exocytosis as well as the vesicle contents in a model cell line, providing direct evidence that zinc regulates neurotransmitter release. To provide insight into how zinc modulates these exocytotic processes, neurotransmitter release and vesicle content were compared with single cell amperometry and intracellular impact vesicle cytometry with a range of zinc concentrations. Additionally, time-of-flight secondary ion mass spectrometry (ToF-SIMS) images of lipid distributions in the cell membrane after zinc treatment correlate to changes in exocytosis. By combining electrochemical techniques and mass spectrometry imaging, we proposed a mechanism by which zinc changes the fusion pore and the rate of neurotransmitter release by changing lipid distributions and results in the modulation of synaptic strength and plasticity.

regulated exocytosis

lipid compostition

zinc

vesicle content

amperometry

Författare

Lin Ren

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Masoumeh Dowlatshahi Pour

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Per Malmberg

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Andrew Ewing

Göteborgs universitet

Chemistry - A European Journal

0947-6539 (ISSN) 1521-3765 (eISSN)

Vol. 25 21 5406-5411

Ämneskategorier

Livsmedelsteknik

Annan kemi

Biofysik

Infrastruktur

Infrastruktur för kemisk avbildning

DOI

10.1002/chem.201900010

Mer information

Senast uppdaterat

2019-05-14