Skin permeation of nickel, cobalt and chromium salts in ex vivo human skin, visualized using mass spectrometry imaging
Journal article, 2021

Skin permeation and distribution of three of the most common skin sensitizers was investigated using a previously developed animal-free exposure method combined with imaging mass spectrometry. Nickel, cobalt, and chromium (III) salts were dissolved in a buffer and exposed to human skin ex vivo, to be analyzed using time of flight secondary ion mass spectrometry (ToF-SIMS). Our findings demonstrate that metal haptens mainly accumulated in the stratum corneum, however all three metal sensitizers could also be detected in the epidermis. Cobalt and chromium (III) species penetrated into the epidermis to a larger extent than nickel species. The degree of penetration into the epidermis is suggested to be affected by the sensitization potency of the metal salts, as well as their speciation, i.e. the amount of the respective metal present in the solution as bioaccessible and solubilised ions. Our method provided permeation profiles in human skin for known sensitizers, on a level of detail that is not possible to achieve by other means. The findings show that the permeation profiles are different, despite these sensitizers being all metal ions and common causes of contact allergy. Studying skin uptake by only considering penetration through the skin might therefore not give accurate results.

Skin sensitizers

Nickel

Cobalt

Chromium

Chemical speciation modelling

Skin permeation

Author

Lina Hagvall

Sahlgrenska University Hospital

University of Gothenburg

Masoumeh Dowlatshahi Pour

University of Gothenburg

Jiabao Feng

Student at Chalmers

Moshtak Karma

University of Gothenburg

Y. Hedberg

Western University

Per Malmberg

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Toxicology in Vitro

0887-2333 (ISSN)

Vol. 76 105232

Subject Categories

Dermatology and Venereal Diseases

Medical Laboratory and Measurements Technologies

Analytical Chemistry

DOI

10.1016/j.tiv.2021.105232

PubMed

34365006

More information

Latest update

8/23/2021