Bioimaging TOF-SIMS: localization of cholesterol in rat kidney sections.
Journal article, 2004
Here, we show the localization of a whole organic molecule in biological tissue using time-of-flight secondary ion mass spectrometry (TOF-SIMS). Rat kidneys were sectioned by cryoultramicrotomy and dried at room temperature. The samples were covered with a thin silver layer and analyzed in an imaging TOF-SIMS instrument equipped with a Ga(+)-source. The cholesterol signal showed a high concentration in the nuclear areas of the epithelial cells and a lower concentration over areas representing the basal lamina of renal tubules. A more diffuse distribution of cholesterol was also found over areas representing the cytoplasm or plasma membrane of the epithelial cells.
Cholesterol
Kidney
Computer-Assisted
Cryoultramicrotomy
chemistry
methods
Animals
Mass
chemistry
Rats
Image Processing
Spectrometry
Male
Microscopy
metabolism
ultrastructure
methods
Secondary Ion
metabolism
Rats
Silver
Sprague-Dawley
Author
Håkan Nygren
University of Gothenburg
Per Malmberg
University of Gothenburg
Christian Kriegeskotte
Heinrich F Arlinghaus
FEBS Letters
0014-5793 (ISSN) 18733468 (eISSN)
Vol. 566 1-3 291-3Subject Categories
MEDICAL AND HEALTH SCIENCES
DOI
10.1016/j.febslet.2004.04.052
PubMed
15147911