Localization of cholesterol, phosphocholine and galactosylceramide in rat cerebellar cortex with imaging TOF-SIMS equipped with a bismuth cluster ion source.
Journal article, 2005
Time-of-flight secondary-ion-mass-spectrometry (TOF-SIMS) was utilized to address the issue of co-localization of cholesterol, phosphocholine and galactosylceramide in rat cerebellar cortex. Rat cerebellum was fixed, freeze-protected by sucrose, frozen and sectioned by cryoultramicrotomy and dried at room temperature. The samples were analyzed in an imaging TOF-SIMS instrument equipped with a Bi(1-7)+-source. The cholesterol signal (m/z 369 and 385) was localized in Purkinje cells and in nuclei of granular layer cells. The phosphocholine headgroup of phosphatidylcholine and sphingomyelin was localized by imaging a specific fragment (m/z 86). This signal was localized in the molecular layer of cerebellar cortex, in Purkinje cells and in parts of the granular layer probably representing the synapse-rich glomeruli. The galactosylceramide was localized by imaging the quasi-molecular ions at m/z 835 and 851, showed a clear colocalization with cholesterol, but also a specific localization in dots (diameter
Rats
Secondary Ion
Cerebellar Cortex
metabolism
Phosphorylcholine
Mass
Animals
Bismuth
metabolism
metabolism
Male
Spectrometry
methods
Tissue Distribution
Sprague-Dawley
Rats
Purkinje Cells
metabolism
metabolism
Cholesterol
Galactosylceramides
Author
Håkan Nygren
University of Gothenburg
Katrin Börner
University of Gothenburg
Birgit Hagenhoff
Per Malmberg
University of Gothenburg
Jan-Eric Månsson
University of Gothenburg
Biochimica et biophysica acta
0006-3002 (ISSN)
Vol. 1737 2-3 102-10Subject Categories
MEDICAL AND HEALTH SCIENCES
DOI
10.1016/j.bbalip.2005.10.004
PubMed
16300993