Spatial neuroproteomics using imaging mass spectrometry
Journal article, 2015

The nervous system constitutes arguably the most complicated and least understood cellular network in the human body. This consequently manifests itself in the fact that the molecular bases of neurodegenerative diseases remain unknown. The limited understanding of neurobiological mechanisms relates directly to the lack of appropriate bioanalytical technologies that allow highly resolved, sensitive, specific and comprehensive molecular imaging in complex biological matrices. Imaging mass spectrometry (IMS) is an emerging technique for molecular imaging. The technique is characterized by its high chemical specificity allowing comprehensive, spatial protein and peptide profiling in situ. Imaging MS represents therefore a powerful approach for investigation of spatio-temporal protein and peptide regulations in CNS derived tissue and cells. This review aims to provide a concise overview of major developments and applications concerning imaging mass spectrometry based protein and peptide profiling in neurobiological and biomedical research. This article is part of a Special Issue entitled: Neuroproteomics: Applications in Neuroscience and Neurology.

ToF-SIMS

Central nervous system (CNS)

MALDI-ToF

Proteomics

Imaging mass spectrometry

Peptidomics

Author

Jörg Hanrieder

University of Gothenburg

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Analytical Chemistry

Per Malmberg

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Analytical Chemistry

Andrew Ewing

University of Gothenburg

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Analytical Chemistry

Biochimica et Biophysica Acta - Proteins and Proteomics

1570-9639 (ISSN)

Vol. 1854 7 718-731

Subject Categories

Biochemistry and Molecular Biology

DOI

10.1016/j.bbapap.2014.12.026

More information

Created

10/7/2017