Spatial neuroproteomics using imaging mass spectrometry
Artikel i vetenskaplig tidskrift, 2015

The nervous system constitutes arguably the most complicated and least understood cellular network in the human body. This consequently manifests itself in the fact that the molecular bases of neurodegenerative diseases remain unknown. The limited understanding of neurobiological mechanisms relates directly to the lack of appropriate bioanalytical technologies that allow highly resolved, sensitive, specific and comprehensive molecular imaging in complex biological matrices. Imaging mass spectrometry (IMS) is an emerging technique for molecular imaging. The technique is characterized by its high chemical specificity allowing comprehensive, spatial protein and peptide profiling in situ. Imaging MS represents therefore a powerful approach for investigation of spatio-temporal protein and peptide regulations in CNS derived tissue and cells. This review aims to provide a concise overview of major developments and applications concerning imaging mass spectrometry based protein and peptide profiling in neurobiological and biomedical research. This article is part of a Special Issue entitled: Neuroproteomics: Applications in Neuroscience and Neurology.

ToF-SIMS

Central nervous system (CNS)

MALDI-ToF

Proteomics

Imaging mass spectrometry

Peptidomics

Författare

Jörg Hanrieder

Göteborgs universitet

Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Per Malmberg

Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Andrew Ewing

Göteborgs universitet

Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Biochimica et Biophysica Acta - Proteins and Proteomics

1570-9639 (ISSN)

Vol. 1854 718-731

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1016/j.bbapap.2014.12.026