Localization of lipids in the aortic wall with imaging TOF-SIMS
Journal article, 2007
Time-of-flight secondary-ion-mass-spectrometry (TOF-SIMS) was utilized to address the issue of localization of lipids and inorganic ions in healthy rat aorta and human atherosclerotic plaque. Pieces of rat aorta were high pressure frozen, freeze-fractured and freeze dried. The samples were analyzed by imaging TOF-SIMS equipped with a Bi(1-7)(+)-source. Reference lipid samples were analyzed and compared to data obtained by analysis of the rat aorta samples. Fatty acids, cholesterol, oxysterol and diacylglycerols were detected and localized. A heterogeneous lipid distribution could be shown in the aorta, where the lamellae of the aorta, distinguished by imaging of CN(-), appeared enriched in cholesterol, oxysterol and diacylglycerols, while the smooth muscle tissue, identified by imaging of PO(3), appeared enriched in phosphocholine. Palmitic/palmitoleic acid and stearic/oleic acid appeared to be heterogeneously distributed over the aorta with high concentration areas located especially in the tunica media region of the aorta. Human atherosclerotic plaque showed an irregular cholesterol distribution mainly located in spots in the intima region with elongated diacylglycerol regions located mainly in the media region.
Sprague-Dawley
Cholesterol/metabolism
Humans
Oleic Acid/metabolism
Fatty Acids
Rats
Palmitic Acid/metabolism
Secondary Ion/*methods
Lipids/*analysis
Aorta/*metabolism/ultrastructure
Freeze Drying
Atherosclerosis/metabolism
Mass
Stearic Acids/metabolism
Spectrometry
Animals
Monounsaturated/metabolism
Rats
Author
Per Malmberg
University of Gothenburg
K. Borner
Yun Chen
University of Gothenburg
Peter Friberg
University of Gothenburg
B. Hagenhoff
Jan-Eric Månsson
Håkan Nygren
University of Gothenburg
Biochim Biophys Acta
0006-3002 (ISSN)
Vol. 1771 2 185-95Subject Categories
MEDICAL AND HEALTH SCIENCES