Localization of lipids in the aortic wall with imaging TOF-SIMS
Artikel i vetenskaplig tidskrift, 2007

Time-of-flight secondary-ion-mass-spectrometry (TOF-SIMS) was utilized to address the issue of localization of lipids and inorganic ions in healthy rat aorta and human atherosclerotic plaque. Pieces of rat aorta were high pressure frozen, freeze-fractured and freeze dried. The samples were analyzed by imaging TOF-SIMS equipped with a Bi(1-7)(+)-source. Reference lipid samples were analyzed and compared to data obtained by analysis of the rat aorta samples. Fatty acids, cholesterol, oxysterol and diacylglycerols were detected and localized. A heterogeneous lipid distribution could be shown in the aorta, where the lamellae of the aorta, distinguished by imaging of CN(-), appeared enriched in cholesterol, oxysterol and diacylglycerols, while the smooth muscle tissue, identified by imaging of PO(3), appeared enriched in phosphocholine. Palmitic/palmitoleic acid and stearic/oleic acid appeared to be heterogeneously distributed over the aorta with high concentration areas located especially in the tunica media region of the aorta. Human atherosclerotic plaque showed an irregular cholesterol distribution mainly located in spots in the intima region with elongated diacylglycerol regions located mainly in the media region.

Sprague-Dawley

Cholesterol/metabolism

Humans

Oleic Acid/metabolism

Fatty Acids

Rats

Palmitic Acid/metabolism

Secondary Ion/*methods

Lipids/*analysis

Aorta/*metabolism/ultrastructure

Freeze Drying

Atherosclerosis/metabolism

Mass

Stearic Acids/metabolism

Spectrometry

Animals

Monounsaturated/metabolism

Rats

Författare

Per Malmberg

Göteborgs universitet

K. Borner

Yun Chen

Göteborgs universitet

Peter Friberg

Göteborgs universitet

B. Hagenhoff

Jan-Eric Månsson

Håkan Nygren

Göteborgs universitet

Biochim Biophys Acta

0006-3002 (ISSN)

Vol. 1771 2 185-95

Ämneskategorier

MEDICIN OCH HÄLSOVETENSKAP