Early interactions between leukocytes and three different potentially bioactive titanium surface modifications
Journal article, 2011
The aim of the present study was to compare the early interactions between leukocytes and three different surface modifications, suggested as bioactive. Blasted titanium discs were modified by alkali and heat treatment, sodium fluoride treatment, or hydroxyapatite coating. A number of these discs were also immersed in simulated body fluid (SBF) for a week, a treatment which yielded high levels of calcium and phosphate on each surface type. The specimens were exposed for human venous blood for 32 minutes and the respiratory burst response was measured in terms of reactive oxygen species with a luminometer, and coverage of viable cells with a fluorescence microscope after staining steps. The topography, morphology, and chemistry of the surfaces were evaluated with optical interferometry and scanning electron microscopy/energy dispersive X-ray analysis (SEM/EDX). A high respiratory burst response was found for HA coated titanium in comparison with the other surface groups (p < 0.0005). The SBF immersion resulted in an increased respiratory burst response (p < 0.0005) and removed statistically significant differences between the surface groups. Thus, the results in the present study indicate that different titanium surface modifications influence the early inflammatory response differently, and that calcium phosphate compounds increase the inflammatory response.
Reactive Oxygen Species
Durapatite
immunology
metabolism
chemistry
metabolism
Sodium Fluoride
Body Fluids
chemistry
Respiratory Burst
metabolism
physiology
Biocompatible
Hot Temperature
Titanium
Coated Materials
chemistry
Materials Testing
Surface Properties
Leukocytes
chemistry
chemistry
Humans
Author
Anna Arvidsson
University of Gothenburg
Per Malmberg
University of Gothenburg
Per Kjellin
Promimic AB
F Currie
Promimic AB
Martin Arvidsson
Chalmers, Technology Management and Economics, Quality Sciences
Victoria Franke Stenport
University of Gothenburg
Journal of Biomedical Materials Research - Part B Applied Biomaterials
1552-4973 (ISSN) 15524981 (eISSN)
Vol. 97 2 364-72Subject Categories
Dentistry
DOI
10.1002/jbm.b.31823
PubMed
21442746