Early interactions between leukocytes and three different potentially bioactive titanium surface modifications
Artikel i vetenskaplig tidskrift, 2011

The aim of the present study was to compare the early interactions between leukocytes and three different surface modifications, suggested as bioactive. Blasted titanium discs were modified by alkali and heat treatment, sodium fluoride treatment, or hydroxyapatite coating. A number of these discs were also immersed in simulated body fluid (SBF) for a week, a treatment which yielded high levels of calcium and phosphate on each surface type. The specimens were exposed for human venous blood for 32 minutes and the respiratory burst response was measured in terms of reactive oxygen species with a luminometer, and coverage of viable cells with a fluorescence microscope after staining steps. The topography, morphology, and chemistry of the surfaces were evaluated with optical interferometry and scanning electron microscopy/energy dispersive X-ray analysis (SEM/EDX). A high respiratory burst response was found for HA coated titanium in comparison with the other surface groups (p < 0.0005). The SBF immersion resulted in an increased respiratory burst response (p < 0.0005) and removed statistically significant differences between the surface groups. Thus, the results in the present study indicate that different titanium surface modifications influence the early inflammatory response differently, and that calcium phosphate compounds increase the inflammatory response.

Surface Properties

chemistry

chemistry

Biocompatible

Hot Temperature

metabolism

Respiratory Burst

metabolism

chemistry

Sodium Fluoride

Coated Materials

Reactive Oxygen Species

Body Fluids

Humans

metabolism

Durapatite

chemistry

immunology

Titanium

Leukocytes

chemistry

physiology

Materials Testing

Författare

Anna Arvidsson

Göteborgs universitet

Per Malmberg

Göteborgs universitet

Per Kjellin

Promimic AB

F Currie

Promimic AB

Martin Arvidsson

Chalmers, Teknikens ekonomi och organisation, Industriell kvalitetsutveckling

Victoria Franke Stenport

Göteborgs universitet

Journal of Biomedical Materials Research - Part B Applied Biomaterials

1552-4973 (ISSN)

Vol. 97 364-72

Ämneskategorier

Odontologi

DOI

10.1002/jbm.b.31823

PubMed

21442746