Bioimaging TOF-SIMS: High resolution 3D imaging of single cells.
Journal article, 2007
The distribution of phosphocholine ions (m/z 184, m/z 86), sodium ions, and potassium ions in thyroid tumor cells was analyzed by imaging TOF-SIMS. Repeated sputtering with a C(60) (+) source and subsequent analysis with a Bi(3) (+) gun produced a series of 138 images that were stacked to make a 3D display of the chemistry of cells. Phosphocholine was seen in the plasma membrane (m/z 184) and intracellular membranes (m/z 86). The different fragmentation of the phospholipid probably reflects the chemical composition of membranes at these sites. High intensity of secondary ion signals of potassium was seen in membrane-encompassed cellular compartments. The data indicate that potassium ions are compartmentalized in thyroid tumor cells.
analysis
analysis
Cell Line
Potassium
Humans
Tumor
Three-Dimensional
analysis
Ions
Phosphorylcholine
Imaging
analysis
Sodium
Mass Spectrometry
Author
Håkan Nygren
University of Gothenburg
Birgit Hagenhoff
Per Malmberg
University of Gothenburg
Mikael Nilsson
University of Gothenburg
Katrin Börner
University of Gothenburg
Microscopy Research and Technique
1059-910X (ISSN) 10970029 (eISSN)
Vol. 70 11 969-74Subject Categories
Cell and Molecular Biology
Other Basic Medicine
DOI
10.1002/jemt.20502
PubMed
17661396