Bioimaging TOF-SIMS: High resolution 3D imaging of single cells.
Artikel i vetenskaplig tidskrift, 2007

The distribution of phosphocholine ions (m/z 184, m/z 86), sodium ions, and potassium ions in thyroid tumor cells was analyzed by imaging TOF-SIMS. Repeated sputtering with a C(60) (+) source and subsequent analysis with a Bi(3) (+) gun produced a series of 138 images that were stacked to make a 3D display of the chemistry of cells. Phosphocholine was seen in the plasma membrane (m/z 184) and intracellular membranes (m/z 86). The different fragmentation of the phospholipid probably reflects the chemical composition of membranes at these sites. High intensity of secondary ion signals of potassium was seen in membrane-encompassed cellular compartments. The data indicate that potassium ions are compartmentalized in thyroid tumor cells.

analysis

analysis

Cell Line

Potassium

Humans

Tumor

Three-Dimensional

analysis

Ions

Phosphorylcholine

Imaging

analysis

Sodium

Mass Spectrometry

Författare

Håkan Nygren

Göteborgs universitet

Birgit Hagenhoff

Per Malmberg

Göteborgs universitet

Mikael Nilsson

Göteborgs universitet

Katrin Börner

Göteborgs universitet

Microscopy Research and Technique

1059-910X (ISSN)

Vol. 70 11 969-74

Ämneskategorier

Cell- och molekylärbiologi

Annan medicinsk grundvetenskap

DOI

10.1002/jemt.20502

PubMed

17661396