Combined Amperometry and Electrochemical Cytometry Reveal Differential Effects of Cocaine and Methyphenidate on Exocytosis and the Fraction of Chemical Release
Artikel i vetenskaplig tidskrift, 2019

Amperometry with nanotip electrodes has been applied to show cocaine and methylphenidate not only trigger declines in vesicle content and exocytotic catecholamine release in a model cell line but also differentially change the fraction of transmitter released from each individual vesicle. In addition, cocaine accelerates exocytotic release dynamics while they remain unchanged after methylphenidate treatment. The parameters from pre-spike feet for the two drugs are also in opposition, suggesting this aspect of release is affected differentially. As cocaine and methylphenidate are psychostimulants with similar pharmacologic action but have opposite effects on cognition, these results might provide a missing link between the regulation of exocytosis and vesicles and the effect of this regulation on cognition, learning, and memory. A speculative chemical mechanism of the effect of these drugs on vesicle content and exocytosis is presented.

exocytosis

vesicles

catecholamines

cocaine

methylphenidate

Författare

Wanying Zhu

Nanjing Medical University

Göteborgs universitet

Chaoyi Gu

Göteborgs universitet

Johan Dunevall

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Lin Ren

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Xuemin Zhou

Nanjing Medical University

Andrew Ewing

Göteborgs universitet

Angewandte Chemie - International Edition

1433-7851 (ISSN) 1521-3773 (eISSN)

Vol. 58 13 4238-4242

Ämneskategorier

Farmaceutisk vetenskap

Neurovetenskaper

Annan kemi

DOI

10.1002/anie.201813717

Mer information

Senast uppdaterat

2019-12-02