Combined Amperometry and Electrochemical Cytometry Reveal Differential Effects of Cocaine and Methyphenidate on Exocytosis and the Fraction of Chemical Release
Journal article, 2019

Amperometry with nanotip electrodes has been applied to show cocaine and methylphenidate not only trigger declines in vesicle content and exocytotic catecholamine release in a model cell line but also differentially change the fraction of transmitter released from each individual vesicle. In addition, cocaine accelerates exocytotic release dynamics while they remain unchanged after methylphenidate treatment. The parameters from pre-spike feet for the two drugs are also in opposition, suggesting this aspect of release is affected differentially. As cocaine and methylphenidate are psychostimulants with similar pharmacologic action but have opposite effects on cognition, these results might provide a missing link between the regulation of exocytosis and vesicles and the effect of this regulation on cognition, learning, and memory. A speculative chemical mechanism of the effect of these drugs on vesicle content and exocytosis is presented.

exocytosis

vesicles

catecholamines

cocaine

methylphenidate

Author

Wanying Zhu

Nanjing Medical University

University of Gothenburg

Chaoyi Gu

University of Gothenburg

Johan Dunevall

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Lin Ren

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Xuemin Zhou

Nanjing Medical University

Andrew Ewing

University of Gothenburg

Angewandte Chemie - International Edition

1433-7851 (ISSN) 1521-3773 (eISSN)

Vol. 58 13 4238-4242

Subject Categories

Pharmaceutical Sciences

Neurosciences

Other Chemistry Topics

DOI

10.1002/anie.201813717

More information

Latest update

12/2/2019