Mass spectrometry imaging of freeze-dried membrane phospholipids of dividing Tetrahymena pyriformis
Artikel i vetenskaplig tidskrift, 2013

Time of Flight secondary ion mass spectrometry (TOF-SIMS) has been used to explore the distribution of phospholipids in the plasma membrane of Tetrahymena pyriformis during cell division. The dividing cells were freeze-dried prior to analysis followed by line scan and region of interest analysis at various stages of cell division. The results showed no signs of phospholipid domain formation at the junction between the dividing cells. Instead the results showed that the sample preparation technique had a great impact on one of the examined phospholipids, namely phosphatidylcholine (PC). Phosphatidylcholine and 2-aminoethylphosphonolipid (2-AEP) have therefore been evaluated in Tetrahymena cells that have been subjected to different sample preparation techniques: freeze drying ex situ, freeze fracture, and freeze fracture with partial or total freeze drying in situ. The result suggests that freeze drying ex situ causes the celia to collapse and cover the plasma membrane.

thermophila

SIMS

samples

phospholipids

Tetrahymena

dividing cells

ciliate

freeze drying

localization

freeze fracture

motility

phosphonolipids

tof-sims

cytokinesis

Författare

Ingela Lanekoff

Göteborgs universitet

Nhu TN Phan

Göteborgs universitet

C. T. Van Bell

Edinboro University

N. Winograd

Pennsylvania State University

Peter Sjövall

Göteborgs universitet

Andrew Ewing

Chalmers, Kemi- och bioteknik, Analytisk kemi

Göteborgs universitet

Surface and Interface Analysis

0142-2421 (ISSN) 1096-9918 (eISSN)

Vol. 45 211-214

Ämneskategorier

Kemi

DOI

10.1002/sia.5017