Mass spectrometry imaging of freeze-dried membrane phospholipids of dividing Tetrahymena pyriformis
Artikel i vetenskaplig tidskrift, 2013

Time of Flight secondary ion mass spectrometry (TOF-SIMS) has been used to explore the distribution of phospholipids in the plasma membrane of Tetrahymena pyriformis during cell division. The dividing cells were freeze-dried prior to analysis followed by line scan and region of interest analysis at various stages of cell division. The results showed no signs of phospholipid domain formation at the junction between the dividing cells. Instead the results showed that the sample preparation technique had a great impact on one of the examined phospholipids, namely phosphatidylcholine (PC). Phosphatidylcholine and 2-aminoethylphosphonolipid (2-AEP) have therefore been evaluated in Tetrahymena cells that have been subjected to different sample preparation techniques: freeze drying ex situ, freeze fracture, and freeze fracture with partial or total freeze drying in situ. The result suggests that freeze drying ex situ causes the celia to collapse and cover the plasma membrane.

phospholipids

phosphonolipids

freeze drying

tof-sims

ciliate

cytokinesis

dividing cells

motility

SIMS

thermophila

freeze fracture

samples

localization

Tetrahymena

Författare

Ingela Lanekoff

Göteborgs universitet

Nhu TN Phan

Göteborgs universitet

C. T. Van Bell

Edinboro University

N. Winograd

Pennsylvania State University

Peter Sjövall

Göteborgs universitet

Andrew Ewing

Chalmers, Kemi- och bioteknik, Analytisk kemi

Göteborgs universitet

Surface and Interface Analysis

0142-2421 (ISSN) 1096-9918 (eISSN)

Vol. 45 1 211-214

Ämneskategorier

Analytisk kemi

Kemi

Miljövetenskap

DOI

10.1002/sia.5017

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Senast uppdaterat

2024-09-05