Mass spectrometry imaging of freeze-dried membrane phospholipids of dividing Tetrahymena pyriformis
Journal article, 2013

Time of Flight secondary ion mass spectrometry (TOF-SIMS) has been used to explore the distribution of phospholipids in the plasma membrane of Tetrahymena pyriformis during cell division. The dividing cells were freeze-dried prior to analysis followed by line scan and region of interest analysis at various stages of cell division. The results showed no signs of phospholipid domain formation at the junction between the dividing cells. Instead the results showed that the sample preparation technique had a great impact on one of the examined phospholipids, namely phosphatidylcholine (PC). Phosphatidylcholine and 2-aminoethylphosphonolipid (2-AEP) have therefore been evaluated in Tetrahymena cells that have been subjected to different sample preparation techniques: freeze drying ex situ, freeze fracture, and freeze fracture with partial or total freeze drying in situ. The result suggests that freeze drying ex situ causes the celia to collapse and cover the plasma membrane.

thermophila

SIMS

samples

phospholipids

Tetrahymena

dividing cells

ciliate

freeze drying

localization

freeze fracture

motility

phosphonolipids

tof-sims

cytokinesis

Author

Ingela Lanekoff

University of Gothenburg

Nhu TN Phan

University of Gothenburg

C. T. Van Bell

Edinboro University

N. Winograd

Pennsylvania State University

Peter Sjövall

University of Gothenburg

Andrew Ewing

Chalmers, Chemical and Biological Engineering, Analytical Chemistry

University of Gothenburg

Surface and Interface Analysis

0142-2421 (ISSN) 1096-9918 (eISSN)

Vol. 45 1 211-214

Subject Categories

Chemical Sciences

DOI

10.1002/sia.5017

More information

Latest update

4/6/2018 1