Electrochemical Investigation of the Interaction between Catecholamines and ATP
Artikel i vetenskaplig tidskrift, 2018

The study of the colligative properties of adenosine 5′-triphosphate (ATP) and catecholamines has received the attention of scientists for decades, as they could explain the capabilities of secretory vesicles (SVs) to accumulate neurotransmitters. In this Article, we have applied electrochemical methods to detect such interactions in vitro, at the acidic pH of SVs (pH 5.5) and examined the effect of compounds having structural similarities that correlate with functional groups of ATP (adenosine, phosphoric acid and sodium phosphate salts) and catecholamines (catechol). Chronoamperometry and fast scan cyclic voltammetry (FSCV) provide evidence compatible with an interaction of the catechol and adenine rings. This interaction is also reinforced by an electrostatic interaction between the phosphate group of ATP and the protonated ammonium group of catecholamines. Furthermore, chronoamperometry data suggest that the presence of ATP subtlety reduces the apparent diffusion coefficient of epinephrine in aqueous media that adds an additional factor leading to a slower rate of catecholamine exocytosis. This adds another plausible mechanism to regulate individual exocytosis events to alter communication.


Zahra Taleat

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Judith Estévez-Herrera

Universidad de la Laguna

José D. Machado

Universidad de la Laguna

Johan Dunevall

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Andrew Ewing

Göteborgs universitet

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Ricardo Borges

Universidad de la Laguna

Analytical Chemistry

0003-2700 (ISSN) 1520-6882 (eISSN)

Vol. 90 3 1601-1607


Fysikalisk kemi

Biokemi och molekylärbiologi

Organisk kemi



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