Electrochemical Investigation of the Interaction between Catecholamines and ATP
Journal article, 2018

The study of the colligative properties of adenosine 5′-triphosphate (ATP) and catecholamines has received the attention of scientists for decades, as they could explain the capabilities of secretory vesicles (SVs) to accumulate neurotransmitters. In this Article, we have applied electrochemical methods to detect such interactions in vitro, at the acidic pH of SVs (pH 5.5) and examined the effect of compounds having structural similarities that correlate with functional groups of ATP (adenosine, phosphoric acid and sodium phosphate salts) and catecholamines (catechol). Chronoamperometry and fast scan cyclic voltammetry (FSCV) provide evidence compatible with an interaction of the catechol and adenine rings. This interaction is also reinforced by an electrostatic interaction between the phosphate group of ATP and the protonated ammonium group of catecholamines. Furthermore, chronoamperometry data suggest that the presence of ATP subtlety reduces the apparent diffusion coefficient of epinephrine in aqueous media that adds an additional factor leading to a slower rate of catecholamine exocytosis. This adds another plausible mechanism to regulate individual exocytosis events to alter communication.

Author

Zahra Taleat

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Judith Estévez-Herrera

University of La Laguna

José D. Machado

University of La Laguna

Johan Dunevall

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Physical Chemistry

Andrew Ewing

University of Gothenburg

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Ricardo Borges

University of La Laguna

Analytical Chemistry

0003-2700 (ISSN) 1520-6882 (eISSN)

Vol. 90 3 1601-1607

Subject Categories

Physical Chemistry

Biochemistry and Molecular Biology

Organic Chemistry

DOI

10.1021/acs.analchem.7b02494

More information

Latest update

2/28/2018