Intact lipid imaging of mouse brain samples: MALDI, nanoparticle-laser desorption ionization, and 40 keV argon cluster secondary ion mass spectrometry
Artikel i vetenskaplig tidskrift, 2016

We have investigated the capability of nanoparticle-assisted laser desorption ionization mass spectrometry (NP-LDI MS), matrix-assisted laser desorption ionization (MALDI) MS, and gas cluster ion beam secondary ion mass spectrometry (GCIB SIMS) to provide maximum information available in lipid analysis and imaging of mouse brain tissue. The use of Au nanoparticles deposited as a matrix for NP-LDI MS is compared to MALDI and SIMS analysis of mouse brain tissue and allows selective detection and imaging of groups of lipid molecular ion species localizing in the white matter differently from those observed using conventional MALDI with improved imaging potential. We demonstrate that high-energy (40 keV) GCIB SIMS can act as a semi-soft ionization method to extend the useful mass range of SIMS imaging to analyze and image intact lipids in biological samples, closing the gap between conventional SIMS and MALDI techniques. The GCIB SIMS allowed the detection of more intact lipid compounds in the mouse brain compared to MALDI with regular organic matrices. The 40 keV GCIB SIMS also produced peaks observed in the NP-LDI analysis, and these peaks were strongly enhanced in intensity by exposure of the sample to trifluororacetic acid (TFA) vapor prior to analysis. These MS techniques for imaging of different types of lipids create a potential overlap and cross point that can enhance the information for imaging lipids in biological tissue sections.

RESOLUTION

BIOLOGICAL TISSUE

Lipids

TOF-SIMS

DESORPTION/IONIZATION

Mass spectrometry imaging

BEAMS

Gas cluster

ALZHEIMERS-DISEASE

DROSOPHILA BRAIN

SIMSMALDI

Nanoparticle-LDI

CELLS

MS

MATRIX

Författare

Amir Saeid Mohammadi

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Nhu TN Phan

Göteborgs universitet

John Fletcher

Göteborgs universitet

Andrew Ewing

Göteborgs universitet

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Analytical and Bioanalytical Chemistry

1618-2642 (ISSN) 1618-2650 (eISSN)

Vol. 408 24 6857-6868

Ämneskategorier

Analytisk kemi

Atom- och molekylfysik och optik

Radiologi och bildbehandling

DOI

10.1007/s00216-016-9812-5

PubMed

27549796