MS/MS analysis and imaging of lipids across Drosophila brain using secondary ion mass spectrometry
Artikel i vetenskaplig tidskrift, 2017

Lipids are abundant biomolecules performing central roles to maintain proper functioning of cells and biological bodies. Due to their highly complex composition, it is critical to obtain information of lipid structures in order to identify particular lipids which are relevant for a biological process or metabolic pathway under study. Among currently available molecular identification techniques, MS/MS in secondary ion mass spectrometry (SIMS) imaging has been of high interest in the bioanalytical community as it allows visualization of intact molecules in biological samples as well as elucidation of their chemical structures. However, there have been few applications using SIMS and MS/MS owing to instrumental challenges for this capability. We performed MS and MS/MS imaging to study the lipid structures of Drosophila brain using the J105 and 40-keV Ar-4000 (+) gas cluster ion source, with the novelty being the use of MS/MS SIMS analysis of intact lipids in the fly brain. Glycerophospholipids were identified by MS/MS profiling. MS/MS was also used to characterize diglyceride fragment ions and to identify them as triacylglyceride fragments. Moreover, MS/MS imaging offers a unique possibility for detailed elucidation of biomolecular distribution with high accuracy based on the ion images of its fragments. This is particularly useful in the presence of interferences which disturb the interpretation of biomolecular localization.

Fly brain

MS/MS

Imaging

Tandemmass spectrometry

Lipids

ToF-SIMS

IMS/MSI

Författare

Nhu TN Phan

Göteborgs universitet

Universitätsmedizin Göttingen

M. Munem

Göteborgs universitet

Andrew Ewing

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

John Fletcher

Göteborgs universitet

Analytical and Bioanalytical Chemistry

1618-2642 (ISSN) 1618-2650 (eISSN)

Vol. 409 16 3923-3932

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1007/s00216-017-0336-4

PubMed

28389914

Mer information

Senast uppdaterat

2018-02-21