Development and Characterization of a Voltammetric Carbon-Fiber Microelectrode pH Sensor
Artikel i vetenskaplig tidskrift, 2010

This work describes the development and characterization of a modified carbon-fiber microelectrode sensor capable of measuring real-time physiological pH changes in biological microenvironments. The reagentless sensor was fabricated under ambient conditions from voltammetric reduction of the diazonium salt Fast Blue RR onto a carbon-fiber surface in aprotic media. Fast-scan cyclic voltammetry was used to probe redox activity of the p-quinone moiety of the surface-bound molecule as a function of pH. In vitro calibration of the sensor in solutions ranging from pH 6.5 to 8.0 resulted in a pH-dependent anodic peak potential response. Flow-injection analysis was used to characterize the modified microelectrode, revealing sensitivity to acidic and basic changes discernible to 0.005 pH units. Furthermore, the modified electrode was used to measure dynamic in vivo pH changes evoked during neurotransmitter release in the central nervous system of the microanalytical model organism Drosophila melanogaster.

SURFACES

ELECTROCHEMICAL REDUCTION

SCAN CYCLIC VOLTAMMETRY

COVALENT MODIFICATION

EPOXY COMPOSITES

BRAIN

DOPAMINE

DIAZONIUM SALTS

DROSOPHILA LARVAE

ELECTRON-TRANSFER KINETICS

Författare

MA Makos

Donna M Omiatek

Andrew Ewing

Göteborgs universitet

ML Heien

Langmuir

07437463 (ISSN) 15205827 (eISSN)

Vol. 26 12 10386-10391

Ämneskategorier

Kemi

DOI

10.1021/la100134r

Mer information

Skapat

2017-10-10