Single Cell Amperometry Reveals Glycocalyx Hinders the Release of Neurotransmitters During Exocytosis
Artikel i vetenskaplig tidskrift, 2013

The diffusional hindrance of the glycocalyx along the cell surface on exocytotic peaks, observed with single cell amperometry, was investigated. Partial digestion of the glycocalyx with neuraminidase led to the observation of faster peaks, as shown by varied peak parameters. This result indicates that diffusion of small molecules in the partially digested glycocalyx is 2.2 faster than in the intact glycocalyx. Similarly, neutralization of the negative charges present in the cell microenvironment led to faster peak kinetics. The analysis of the vesicular efflux indicates that the diffusion coefficient of dopamine at the cell surface is at most 45% of the diffusion coefficient in free solution. This study shows that the glycocalyx plays an important role in the diffusion kinetics of processes along the cell surface, including exocytotic events.

fusion pore

sialic-acid

endothelial-cells

pc12 cells

chemical-analysis

extracellular-matrix

to-substrate contacts

electrophoretic mobility

adrenal chromaffin cells

vesicular volume

Författare

R. Trouillon

Göteborgs universitet

Andrew Ewing

Chalmers, Kemi- och bioteknik, Analytisk kemi

Göteborgs universitet

Analytical Chemistry

0003-2700 (ISSN) 1520-6882 (eISSN)

Vol. 85 9 4822-4828

Ämneskategorier

Analytisk kemi

DOI

10.1021/ac4008682