Single Cell Amperometry Reveals Glycocalyx Hinders the Release of Neurotransmitters During Exocytosis
Journal article, 2013

The diffusional hindrance of the glycocalyx along the cell surface on exocytotic peaks, observed with single cell amperometry, was investigated. Partial digestion of the glycocalyx with neuraminidase led to the observation of faster peaks, as shown by varied peak parameters. This result indicates that diffusion of small molecules in the partially digested glycocalyx is 2.2 faster than in the intact glycocalyx. Similarly, neutralization of the negative charges present in the cell microenvironment led to faster peak kinetics. The analysis of the vesicular efflux indicates that the diffusion coefficient of dopamine at the cell surface is at most 45% of the diffusion coefficient in free solution. This study shows that the glycocalyx plays an important role in the diffusion kinetics of processes along the cell surface, including exocytotic events.

fusion pore



pc12 cells



to-substrate contacts

electrophoretic mobility

adrenal chromaffin cells

vesicular volume


R. Trouillon

University of Gothenburg

Andrew Ewing

Chalmers, Chemical and Biological Engineering, Analytical Chemistry

University of Gothenburg

Analytical Chemistry

0003-2700 (ISSN) 1520-6882 (eISSN)

Vol. 85 9 4822-4828

Subject Categories

Analytical Chemistry



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