Single cell amperometry reveals curcuminoids modulate the release of neurotransmitters during exocytosis from PC12 cells
Artikel i vetenskaplig tidskrift, 2016

We used single cell amperometry to examine whether curcumin and bisdemethoxycurcumin (BDMC), substances that are suggested to affect learning and memory, can modulate monoamine release from PC12 cells. Our results indicate both curcumin and BDMC need long-term treatment (72 h in this study) to influence exocytosis effectively. By analyzing the parameters calculated from single exocytosis events, it can be concluded that curcumin and BDMC affect exocytosis through different mechanisms. Curcumin accelerates the event dynamics with no significant change of the monoamine amount released from single exocytotic events, whereas BDMC attenuates the amount from single exocytotic event with no significant change of the event dynamics. This comparison of the effect of curcumin and BDMC on exocytosis at the single cell level brings insight into their different mechanisms, which might lead to different biological actions. The effect of curcumin and BDMC on the opening and closing of the exocytotic fusion pore were also investigated. These results might be helpful for understanding the improvement of learning and memory and the anti-depression properties of curcuminoids.

Curcumin

Model cells

Bisdemethoxycurcumin

Single cell amperometry

Exocytosis

PC12 cells

Författare

Li Xianchan

Göteborgs universitet

Amir Saeid Mohammadi

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Andrew Ewing

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Journal of Electroanalytical Chemistry

0022-0728 (ISSN)

Vol. 781 30-35

Ämneskategorier

Oorganisk kemi

DOI

10.1016/j.jelechem.2016.10.025

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2018-02-21