Actin Controls the Vesicular Fraction of Dopamine Released During Extended Kiss and Run Exocytosis
Artikel i vetenskaplig tidskrift, 2014
The effect of latrunculin A, an inhibitor of actin cross-linking, on exocytosis in PC12 cells was investigated with single cell amperometry. This analysis strongly suggests that the actin cytoskeleton might be involved in regulating exocytosis, especially by mediating the constriction of the pore. In an extended kiss-and-run release mode, actin could actually control the fraction of neurotransmitters released by the vesicle. This scaffold appears to contribute, with the lipid membrane and the protein machinery, to the closing dynamics of the pore, in competition with other forces mediating the opening of the exocytotic channel.
MICROELECTRODE ARRAYS
CHROMAFFIN
DENSE-CORE VESICLES
NEUROTRANSMITTER RELEASE
QUANTAL SIZE
CELLS
REGULATED EXOCYTOSIS
AMPEROMETRIC DETECTION
SINGLE VESICLE RELEASE
FUSION PORE
CHEMICAL-ANALYSIS
Författare
R. Trouillon
Göteborgs universitet
Andrew Ewing
Chalmers, Kemi- och bioteknik, Analytisk kemi
Göteborgs universitet
ACS Chemical Biology
1554-8929 (ISSN) 1554-8937 (eISSN)
Vol. 9 3 812-820Ämneskategorier
Cell- och molekylärbiologi
DOI
10.1021/cb400665f