Actin Controls the Vesicular Fraction of Dopamine Released During Extended Kiss and Run Exocytosis
Artikel i vetenskaplig tidskrift, 2014

The effect of latrunculin A, an inhibitor of actin cross-linking, on exocytosis in PC12 cells was investigated with single cell amperometry. This analysis strongly suggests that the actin cytoskeleton might be involved in regulating exocytosis, especially by mediating the constriction of the pore. In an extended kiss-and-run release mode, actin could actually control the fraction of neurotransmitters released by the vesicle. This scaffold appears to contribute, with the lipid membrane and the protein machinery, to the closing dynamics of the pore, in competition with other forces mediating the opening of the exocytotic channel.

MICROELECTRODE ARRAYS

CHROMAFFIN

DENSE-CORE VESICLES

NEUROTRANSMITTER RELEASE

QUANTAL SIZE

CELLS

REGULATED EXOCYTOSIS

AMPEROMETRIC DETECTION

SINGLE VESICLE RELEASE

FUSION PORE

CHEMICAL-ANALYSIS

Författare

R. Trouillon

Göteborgs universitet

Andrew Ewing

Chalmers, Kemi- och bioteknik, Analytisk kemi

Göteborgs universitet

ACS Chemical Biology

1554-8929 (ISSN) 1554-8937 (eISSN)

Vol. 9 812-820

Ämneskategorier

Cell- och molekylärbiologi

DOI

10.1021/cb400665f