Actin Controls the Vesicular Fraction of Dopamine Released During Extended Kiss and Run Exocytosis
Journal article, 2014
The effect of latrunculin A, an inhibitor of actin cross-linking, on exocytosis in PC12 cells was investigated with single cell amperometry. This analysis strongly suggests that the actin cytoskeleton might be involved in regulating exocytosis, especially by mediating the constriction of the pore. In an extended kiss-and-run release mode, actin could actually control the fraction of neurotransmitters released by the vesicle. This scaffold appears to contribute, with the lipid membrane and the protein machinery, to the closing dynamics of the pore, in competition with other forces mediating the opening of the exocytotic channel.
SINGLE VESICLE RELEASE
MICROELECTRODE ARRAYS
DENSE-CORE VESICLES
QUANTAL SIZE
REGULATED EXOCYTOSIS
FUSION PORE
CELLS
NEUROTRANSMITTER RELEASE
CHROMAFFIN
CHEMICAL-ANALYSIS
AMPEROMETRIC DETECTION
Author
R. Trouillon
University of Gothenburg
Andrew Ewing
Chalmers, Chemical and Biological Engineering, Analytical Chemistry
University of Gothenburg
ACS Chemical Biology
1554-8929 (ISSN) 1554-8937 (eISSN)
Vol. 9 3 812-820Subject Categories
Cell and Molecular Biology
DOI
10.1021/cb400665f