Electrochemical Measurements of Optogenetically Stimulated Quantal Amine Release from Single Nerve Cell Varicosities in Drosophila Larvae
Artikel i vetenskaplig tidskrift, 2015

The nerve terminals found in the body wall of Drosophila melanogaster larvae are readily accessible to experimental manipulation. We used the light-activated ion channel, channelrhodopsin-2, which is expressed by genetic manipulation in TypeII varicosities to study octopamine release in Drosophila. We report the development of a method to measure neurotransmitter release from exocytosis events at individual varicosities in the Drosophila larval system by amperometry. A microelectrode was placed in a region of the muscle containing a varicosity and held at a potential sufficient to oxidize octopamine and the terminal stimulated by blue light. Optical stimulation of TypeII boutons evokes exocytosis of octopamine, which is detected through oxidization at the electrode surface. We observe 22700 +/- 4200 molecules of octopamine released per vesicle. This system provides a genetically accessible platform to study the regulation of amine release at an intact synapse.

octopamine

channelrhodopsin

Drosophila larvae

varicosity

amperometry

Författare

Soodabeh Majdi

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

E. C. Berglund

Göteborgs universitet

Johan Dunevall

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

A. I. Oleinick

Processus d'Activation Selective Par Transfert d'Energie Uni-Electronique ou Radiatif

C. Amatore

Processus d'Activation Selective Par Transfert d'Energie Uni-Electronique ou Radiatif

D. E. Krantz

David Geffen School of Medicine at UCLA

Andrew Ewing

Göteborgs universitet

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Angewandte Chemie - International Edition

1433-7851 (ISSN) 1521-3773 (eISSN)

Vol. 54 13609-13612

Ämneskategorier

Cellbiologi

Biologiska vetenskaper

DOI

10.1002/anie.201506743