Electrochemical Measurements of Optogenetically Stimulated Quantal Amine Release from Single Nerve Cell Varicosities in Drosophila Larvae
Journal article, 2015

The nerve terminals found in the body wall of Drosophila melanogaster larvae are readily accessible to experimental manipulation. We used the light-activated ion channel, channelrhodopsin-2, which is expressed by genetic manipulation in TypeII varicosities to study octopamine release in Drosophila. We report the development of a method to measure neurotransmitter release from exocytosis events at individual varicosities in the Drosophila larval system by amperometry. A microelectrode was placed in a region of the muscle containing a varicosity and held at a potential sufficient to oxidize octopamine and the terminal stimulated by blue light. Optical stimulation of TypeII boutons evokes exocytosis of octopamine, which is detected through oxidization at the electrode surface. We observe 22700 +/- 4200 molecules of octopamine released per vesicle. This system provides a genetically accessible platform to study the regulation of amine release at an intact synapse.

octopamine

channelrhodopsin

Drosophila larvae

varicosity

amperometry

Author

Soodabeh Majdi

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Analytical Chemistry

E. C. Berglund

University of Gothenburg

Johan Dunevall

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Analytical Chemistry

A. I. Oleinick

Processus d'Activation Selective Par Transfert d'Energie Uni-Electronique ou Radiatif

C. Amatore

Processus d'Activation Selective Par Transfert d'Energie Uni-Electronique ou Radiatif

D. E. Krantz

David Geffen School of Medicine at UCLA

Andrew Ewing

University of Gothenburg

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Analytical Chemistry

Angewandte Chemie - International Edition

1433-7851 (ISSN) 1521-3773 (eISSN)

Vol. 54 46 13609-13612

Subject Categories

Cell Biology

Biological Sciences

DOI

10.1002/anie.201506743

More information

Created

10/8/2017