DMSO Chemically Alters Cell Membranes to Slow Exocytosis and Increase the Fraction of Partial Transmitter Released
Artikel i vetenskaplig tidskrift, 2017

Dimethyl sulfoxide (DMSO) is frequently used as a solvent in biological studies and as a vehicle for drug therapy; but the side effects of DMSO, especially on the cell environment, are not well understood, and controls with DMSO are not neutral at higher concentrations. Herein, electrochemical measurement techniques are applied to show that DMSO increases exocytotic neurotransmitter release, while leaving vesicular contents unchanged. In addition, the kinetics of release from DMSOtreated cells are faster than that of untreated ones. The results suggest that DMSO has a significant influence on the chemistry of the cell membrane, leading to alteration of exocytosis. A speculative chemical mechanism of the effect on the fusion pore during exocytosis is presented.

exocytosis

vesicles

electrochemistry

membranes

solvent effect

Författare

Soodabeh Majdi

Göteborgs universitet

Neda Najafinobar

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Johan Dunevall

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Jelena Lovric

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Andrew Ewing

Göteborgs universitet

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

ChemBioChem

1439-4227 (ISSN) 1439-7633 (eISSN)

Vol. 18 19 1898-1902

Ämneskategorier

Oorganisk kemi

Cellbiologi

Biofysik