Ruthenium(II) Complex Enantiomers as Cellular Probes for Diastereomeric Interactions in Confocal and Fluorescence Lifetime Imaging Microscopy
Artikel i vetenskaplig tidskrift, 2011

Ruthenium dipyridophenazine (dppz) complexes are sensitive luminescent probes for hydrophobic environments. Here, we apply multi-frequency fluorescence lifetime imaging microscopy (FLLM) to Delta and Lambda enantiomers of lipophilic ruthenium dppz complexes in live and fixed cells, and their different lifetime staining patterns are related to conventional intensity-based microscopy. Excited-state lifetimes of the enantiomers determined from FLIM measurements correspond well with spectroscopically measured emission decay curves in pure microenvironments of DNA, phospholipid membranes, or a model protein. We show that FLIM can be applied to monitor the long-lived excited states of ruthenium complex enantiomers and, combined with confocal microscopy, give new insight into their biomolecular binding and reveal differences in the microenvironment probed by the complexes.

dipyridophenazine complexes

mechanism

membrane

picosecond

light-switch

solvents

polypyridyl complex

linear dichroism

dna-binding geometries

Författare

Frida Svensson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Maria Abrahamsson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

N. Stromberg

SP Sveriges Tekniska Forskningsinstitut

Andrew Ewing

Göteborgs universitet

Per Lincoln

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Journal of Physical Chemistry Letters

1948-7185 (eISSN)

Vol. 2 397-401

Styrkeområden

Nanovetenskap och nanoteknik

Ämneskategorier

Fysikalisk kemi

DOI

10.1021/jz101580e