Ruthenium(II) Complex Enantiomers as Cellular Probes for Diastereomeric Interactions in Confocal and Fluorescence Lifetime Imaging Microscopy
Journal article, 2011

Ruthenium dipyridophenazine (dppz) complexes are sensitive luminescent probes for hydrophobic environments. Here, we apply multi-frequency fluorescence lifetime imaging microscopy (FLLM) to Delta and Lambda enantiomers of lipophilic ruthenium dppz complexes in live and fixed cells, and their different lifetime staining patterns are related to conventional intensity-based microscopy. Excited-state lifetimes of the enantiomers determined from FLIM measurements correspond well with spectroscopically measured emission decay curves in pure microenvironments of DNA, phospholipid membranes, or a model protein. We show that FLIM can be applied to monitor the long-lived excited states of ruthenium complex enantiomers and, combined with confocal microscopy, give new insight into their biomolecular binding and reveal differences in the microenvironment probed by the complexes.

dipyridophenazine complexes

picosecond

solvents

light-switch

mechanism

polypyridyl complex

linear dichroism

dna-binding geometries

membrane

Author

Frida Svensson

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Maria Abrahamsson

Chalmers, Chemical and Biological Engineering, Physical Chemistry

N. Stromberg

SP Sveriges Tekniska Forskningsinstitut AB

Andrew Ewing

University of Gothenburg

Per Lincoln

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Journal of Physical Chemistry Letters

1948-7185 (eISSN)

Vol. 2 5 397-401

Areas of Advance

Nanoscience and Nanotechnology (2010-2017)

Subject Categories

Physical Chemistry

DOI

10.1021/jz101580e

More information

Latest update

9/6/2018 1