Gas-chromatographic analysis of sugars and related hydroxy acids as acyclic oxime and ester trimethylsilyl derivatives
Journal article, 1974

A g.l.c. method which makes possible the simultaneous analysis of the common types of acidic and neutral sugars is described. Particular advantages are achieved with hydroxy acids, ketoses, and uronic acids. Acids and lactones are first converted into their sodium salts. Free or potential aldehydo and keto groups are transformed into oximes or O-methyloximes. Fully substituted acyclic ester and oxime trimethylsilyl derivatives are prepared with bis(trimethylsilyl)trifluoroacetamide and chlorotrimethylsilane. Syn and anti isomers of the oximes are formed, but a stationary phase can often be chosen on which the two peaks coincide. The silicone stationary phases OV-1, OV-17, QF-1, and XE-60 were used. Retention data are given for aldonic and deoxyaldonic acids, aldoses, ketoses, and uronic acids. Relationships between structure and retention are discussed. The derivatives are suited to structural analysis by gas chromatography—mass spectrometry.

gas chromatography

Me3Si derivatives

hydroxy acids

silicone phases

mass spectra

monosaccharides

oximes

Author

Göran Petersson

Department of Engineering Chemistry

Carbohydrate Research

Vol. 33 47-61

Subject Categories

Analytical Chemistry

DOI

10.1016/S0008-6215(00)82939-0

More information

Created

10/6/2017