Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements
Journal article, 2005

Dual-color cross-correlation spectroscopy allows the detection and quantification of labeled biomolecules at ultra-low concentrations, whereby the sensitivity of the assay correlates with the measurement time. We now describe a parallel multifocal dual-color spectroscopic configuration employing multiple avalanche photodiodes and hardware correlators. Cross-correlation curves are obtained from several dual-color excitation foci simultaneously. Multifocal dual-color excitation is achieved by splitting each of two laser beams (488 and 633 nm) into four sub-beams with the help of two 2 × 2 fan-out diffractive optical elements (DOEs), and subsequent superposition of the two sets of four foci. The fluorescence emission from double-labeled biomolecules is detected by two 2 × 2 fiber arrays.

Author

M. Gösch

Swiss Federal Institute of Technology in Lausanne (EPFL)

Karolinska Institutet

H. Blom

Karolinska Institutet

Swiss Federal Institute of Technology in Lausanne (EPFL)

S. Anderegg

Swiss Federal Institute of Technology in Lausanne (EPFL)

Karolinska Institutet

K. Korn

Karolinska Institutet

Swiss Federal Institute of Technology in Lausanne (EPFL)

P. Thyberg

Swiss Federal Institute of Technology in Lausanne (EPFL)

Karolinska Institutet

M. Wells

Swiss Federal Institute of Technology in Lausanne (EPFL)

Karolinska Institutet

T. Lasser

Karolinska Institutet

Swiss Federal Institute of Technology in Lausanne (EPFL)

R. Rigler

Swiss Federal Institute of Technology in Lausanne (EPFL)

Karolinska Institutet

A. Magnusson

Chalmers

Sverker Hård

Chalmers, Microtechnology and Nanoscience (MC2), Photonics

Journal of Biomedical Optics

1083-3668 (ISSN)

Vol. 10 5 054008

Subject Categories

Electrical Engineering, Electronic Engineering, Information Engineering

DOI

10.1117/1.2080707

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4/5/2022 6