Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements
Journal article, 2005
Dual-color cross-correlation spectroscopy allows the detection and quantification of labeled biomolecules at ultra-low concentrations, whereby the sensitivity of the assay correlates with the measurement time. We now describe a parallel multifocal dual-color spectroscopic configuration employing multiple avalanche photodiodes and hardware correlators. Cross-correlation curves are obtained from several dual-color excitation foci simultaneously. Multifocal dual-color excitation is achieved by splitting each of two laser beams (488 and 633 nm) into four sub-beams with the help of two 2 × 2 fan-out diffractive optical elements (DOEs), and subsequent superposition of the two sets of four foci. The fluorescence emission from double-labeled biomolecules is detected by two 2 × 2 fiber arrays.
Author
M. Gösch
Swiss Federal Institute of Technology in Lausanne (EPFL)
Karolinska Institutet
H. Blom
Karolinska Institutet
Swiss Federal Institute of Technology in Lausanne (EPFL)
S. Anderegg
Swiss Federal Institute of Technology in Lausanne (EPFL)
Karolinska Institutet
K. Korn
Karolinska Institutet
Swiss Federal Institute of Technology in Lausanne (EPFL)
P. Thyberg
Swiss Federal Institute of Technology in Lausanne (EPFL)
Karolinska Institutet
M. Wells
Swiss Federal Institute of Technology in Lausanne (EPFL)
Karolinska Institutet
T. Lasser
Karolinska Institutet
Swiss Federal Institute of Technology in Lausanne (EPFL)
R. Rigler
Swiss Federal Institute of Technology in Lausanne (EPFL)
Karolinska Institutet
A. Magnusson
Chalmers
Sverker Hård
Chalmers, Microtechnology and Nanoscience (MC2), Photonics
Published in
Journal of Biomedical Optics
1083-3668 (ISSN) 15602281 (eISSN)
Vol. 10 Issue 5 art. no 054008Categorizing
Subject Categories (SSIF 2011)
Electrical Engineering, Electronic Engineering, Information Engineering
Identifiers
DOI
10.1117/1.2080707