Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements
Artikel i vetenskaplig tidskrift, 2005

Dual-color cross-correlation spectroscopy allows the detection and quantification of labeled biomolecules at ultra-low concentrations, whereby the sensitivity of the assay correlates with the measurement time. We now describe a parallel multifocal dual-color spectroscopic configuration employing multiple avalanche photodiodes and hardware correlators. Cross-correlation curves are obtained from several dual-color excitation foci simultaneously. Multifocal dual-color excitation is achieved by splitting each of two laser beams (488 and 633 nm) into four sub-beams with the help of two 2 × 2 fan-out diffractive optical elements (DOEs), and subsequent superposition of the two sets of four foci. The fluorescence emission from double-labeled biomolecules is detected by two 2 × 2 fiber arrays.

Författare

M. Gösch

Karolinska Institutet

Ecole Polytechnique Federale de Lausanne

H. Blom

Karolinska Institutet

Ecole Polytechnique Federale de Lausanne

S. Anderegg

Ecole Polytechnique Federale de Lausanne

Karolinska Institutet

K. Korn

Karolinska Institutet

Ecole Polytechnique Federale de Lausanne

P. Thyberg

Ecole Polytechnique Federale de Lausanne

Karolinska Institutet

M. Wells

Karolinska Institutet

Ecole Polytechnique Federale de Lausanne

T. Lasser

Ecole Polytechnique Federale de Lausanne

Karolinska Institutet

R. Rigler

Karolinska Institutet

Ecole Polytechnique Federale de Lausanne

A. Magnusson

Chalmers University of Technology

Sverker Hård

Chalmers, Mikroteknologi och nanovetenskap (MC2), Fotonik

Journal of Biomedical Optics

1083-3668 (ISSN)

Vol. 10

Ämneskategorier

Elektroteknik och elektronik

DOI

10.1117/1.2080707