Lipase-catalyzed Reactions at Interfaces of Two-phase Systems and Microemulsions
Journal article, 2009
This work describes the influence of two polar lipids, Sn-1/3 and Sn-2 monopalmitin, on the activity of lipase in biphasic systems and in microemulsions. In previous communications, we have shown that Sn-2 monoglycerides can replace Sn-1,3 regiospecific lipases at the oil-water interface, causing a drastically reduced rate of lipolysis. We here demonstrate that even if the lipase is expelled from the interface, it can catalyze esterification of the Sn-2 monoglyceride with fatty acids in both macroscopic oil-water systems and in microemulsions, leading to formation of di- and triglycerides.
dynamics
oil
adsorption layers
Synthesis
Lipase
Esterification
surface dilational rheology
esterification
protein films
air/water interface
Interface
water
fluid interfaces
monoglycerides
Author
Pedro Reis
Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry
Reinhard Miller
Max Planck Society
Martin Leser
Nestle S.A.
Heribert Watzke
Nestle S.A.
Applied Biochemistry and Biotechnology
0273-2289 (ISSN) 15590291 (eISSN)
Vol. 158 3 706-721Subject Categories (SSIF 2011)
Physical Chemistry
DOI
10.1007/s12010-008-8354-5