Lipase-catalyzed Reactions at Interfaces of Two-phase Systems and Microemulsions
Artikel i vetenskaplig tidskrift, 2009

This work describes the influence of two polar lipids, Sn-1/3 and Sn-2 monopalmitin, on the activity of lipase in biphasic systems and in microemulsions. In previous communications, we have shown that Sn-2 monoglycerides can replace Sn-1,3 regiospecific lipases at the oil-water interface, causing a drastically reduced rate of lipolysis. We here demonstrate that even if the lipase is expelled from the interface, it can catalyze esterification of the Sn-2 monoglyceride with fatty acids in both macroscopic oil-water systems and in microemulsions, leading to formation of di- and triglycerides.

dynamics

oil

adsorption layers

Synthesis

Lipase

Esterification

surface dilational rheology

esterification

protein films

air/water interface

Interface

water

fluid interfaces

monoglycerides

Författare

Pedro Reis

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

Reinhard Miller

Max Planck-institutet

Martin Leser

Nestle S.A.

Heribert Watzke

Nestle S.A.

Applied Biochemistry and Biotechnology

0273-2289 (ISSN)

Vol. 158 706-721

Ämneskategorier

Fysikalisk kemi

DOI

10.1007/s12010-008-8354-5