Lipase-catalyzed Reactions at Interfaces of Two-phase Systems and Microemulsions
Artikel i vetenskaplig tidskrift, 2009
This work describes the influence of two polar lipids, Sn-1/3 and Sn-2 monopalmitin, on the activity of lipase in biphasic systems and in microemulsions. In previous communications, we have shown that Sn-2 monoglycerides can replace Sn-1,3 regiospecific lipases at the oil-water interface, causing a drastically reduced rate of lipolysis. We here demonstrate that even if the lipase is expelled from the interface, it can catalyze esterification of the Sn-2 monoglyceride with fatty acids in both macroscopic oil-water systems and in microemulsions, leading to formation of di- and triglycerides.
dynamics
oil
adsorption layers
Synthesis
Lipase
Esterification
surface dilational rheology
esterification
protein films
air/water interface
Interface
water
fluid interfaces
monoglycerides
Författare
Pedro Reis
Chalmers, Kemi- och bioteknik, Teknisk ytkemi
Reinhard Miller
Max-Planck-Gesellschaft
Martin Leser
Nestle S.A.
Heribert Watzke
Nestle S.A.
Applied Biochemistry and Biotechnology
0273-2289 (ISSN) 15590291 (eISSN)
Vol. 158 3 706-721Ämneskategorier (SSIF 2011)
Fysikalisk kemi
DOI
10.1007/s12010-008-8354-5