Protein Isolation from Gutted Herring (Clupea harengus) Using pH-Shift Processes
Journal article, 2010
Herring (Clupea harengus) and other pelagic fish species are mainly used for fish meal and oil production and not for human consumption. In this study, acid pH-shift processing and alkaline pH-shift processing were used to isolate proteins from whole gutted herring with the aim to investigate the potential use of herring proteins as a food ingredient. The acid and alkaline processes gave rise to similar protein yields, 59.3 and 57.3%. The protein isolates from both processes had a significantly (p < 0.05) whiter color and higher protein and lower lipid contents than the starting material. The removal of ash was >80% for both processes, with a trend (p = 0.07) toward higher removal during the alkaline process. Also, Ca and Mg removal was significantly (p < 0.05) higher during the alkaline process. The isolated proteins from the acid process contained myosin degradation products and had a lower salt solubility than proteins from the alkaline process. Both protein isolates had an amino acid profile meeting the recommendations for adults according to FAO/WHO/UNU and could produce a surimi gel of medium strength. The results show that pH-shift processing could be a valuable method for the production of functional food proteins from gutted herring.
precipitation
functional-properties
Herring
isoelectric
solubilization
functionality
acid
alkaline
muscle proteins
light muscle
additives
recovery
protein
lipid oxidation
acid
solubilization/precipitation
alkaline solubilization process
cod muscle
Author
Sofia Marmon
Chalmers, Chemical and Biological Engineering, Life Sciences
Ingrid Undeland
Chalmers, Chemical and Biological Engineering, Life Sciences
Journal of Agricultural and Food Chemistry
0021-8561 (ISSN) 1520-5118 (eISSN)
Vol. 58 19 10480-10486Driving Forces
Sustainable development
Subject Categories
Biochemistry and Molecular Biology
Food Science
Areas of Advance
Life Science Engineering (2010-2018)
DOI
10.1021/jf101057q