STRUCTURAL CHARACTERIZATION OF PNA-DNA DUPLEXES BY NMR - EVIDENCE FOR DNA IN A B-LIKE CONFORMATION
Journal article, 1994
The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by H-1 NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.
spectra
thymine-substituted polyamide
assignment
spectroscopy
proton resonances
nuclear magnetic-resonance
strand-displacement
suppression
oligonucleotides
coupling-constants
Author
M. Leijon
A. Graslund
P. E. Nielsen
O. Buchardt
Bengt Nordén
Department of Physical Chemistry
S. M. Kristensen
M. Eriksson
Biochemistry
0006-2960 (ISSN) 1520-4995 (eISSN)
Vol. 33 33 9820-9825Subject Categories
Biochemistry and Molecular Biology
DOI
10.1021/bi00199a002