STRUCTURAL CHARACTERIZATION OF PNA-DNA DUPLEXES BY NMR - EVIDENCE FOR DNA IN A B-LIKE CONFORMATION
Artikel i vetenskaplig tidskrift, 1994

The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by H-1 NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.

spectra

thymine-substituted polyamide

assignment

spectroscopy

proton resonances

nuclear magnetic-resonance

strand-displacement

suppression

oligonucleotides

coupling-constants

Författare

M. Leijon

A. Graslund

P. E. Nielsen

O. Buchardt

Bengt Nordén

Institutionen för fysikalisk kemi

S. M. Kristensen

M. Eriksson

Biochemistry

0006-2960 (ISSN) 1520-4995 (eISSN)

Vol. 33 33 9820-9825

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1021/bi00199a002