DYNAMICS OF BENZO A PYRENE DIOL EPOXIDE ADDUCTS IN POLY(DG-DC) . (DG-DC) STUDIED BY SYNCHROTRON EXCITED FLUORESCENCE POLARIZATION ANISOTROPY DECAY
Journal article, 1992

Time-resolved fluorescence studies have been performed on (+)-anti-7,8-dihydrodiol-9,10-epoxy-benzo[a]pyrene adducts in double-stranded poly(dG-dC) . (dG-dC). Part of the adduct population gives rise to excimer fluorescence. The heterogeneous fluorescence emission decay curves at 22-degrees-C could be resolved into three components with lifetimes: 0.4 ns, 3 ns and 24 ns for the total fluorescence (monomer and excimer emission), and 0.5 ns, 5 ns and 24 ns, respectively, for excimer emission alone. The relative amplitudes for the longer lifetimes were larger for the pure excimer population than for the mixed population. The fluorescence polarization anisotropy decay curves were resolved into two components of rotational correlation times: 0.4 ns and 25 ns for the total fluorescence and 0.3 ns and 33 ns for the excimer fluorescence. We interpret the two rotational correlation times to correspond to local motion of the adduct and segmental motion of the polynucleotide, respectively.

poly(dg-dc).(dg-dc)

anisotropy decay

molecular-dynamics

fluorescence lifetimes

synchrotron radiation

dna

binding

benzo[a]pyrene diol epoxide

Author

A. Graslund

Seog K. Kim

Department of Physical Chemistry

Svante Eriksson

Department of Physical Chemistry

Bengt Nordén

Department of Physical Chemistry

B. Jernstrom

Biophysical Chemistry

0301-4622 (ISSN)

Vol. 44 1 21-28

Subject Categories

Biochemistry and Molecular Biology

DOI

10.1016/0301-4622(92)85032-y

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Created

10/6/2017