DYNAMICS OF BENZO A PYRENE DIOL EPOXIDE ADDUCTS IN POLY(DG-DC) . (DG-DC) STUDIED BY SYNCHROTRON EXCITED FLUORESCENCE POLARIZATION ANISOTROPY DECAY
Artikel i vetenskaplig tidskrift, 1992

Time-resolved fluorescence studies have been performed on (+)-anti-7,8-dihydrodiol-9,10-epoxy-benzo[a]pyrene adducts in double-stranded poly(dG-dC) . (dG-dC). Part of the adduct population gives rise to excimer fluorescence. The heterogeneous fluorescence emission decay curves at 22-degrees-C could be resolved into three components with lifetimes: 0.4 ns, 3 ns and 24 ns for the total fluorescence (monomer and excimer emission), and 0.5 ns, 5 ns and 24 ns, respectively, for excimer emission alone. The relative amplitudes for the longer lifetimes were larger for the pure excimer population than for the mixed population. The fluorescence polarization anisotropy decay curves were resolved into two components of rotational correlation times: 0.4 ns and 25 ns for the total fluorescence and 0.3 ns and 33 ns for the excimer fluorescence. We interpret the two rotational correlation times to correspond to local motion of the adduct and segmental motion of the polynucleotide, respectively.

poly(dg-dc).(dg-dc)

anisotropy decay

molecular-dynamics

fluorescence lifetimes

synchrotron radiation

dna

binding

benzo[a]pyrene diol epoxide

Författare

A. Graslund

Seog K. Kim

Institutionen för fysikalisk kemi

Svante Eriksson

Institutionen för fysikalisk kemi

Bengt Nordén

Institutionen för fysikalisk kemi

B. Jernstrom

Biophysical Chemistry

0301-4622 (ISSN)

Vol. 44 1 21-28

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1016/0301-4622(92)85032-y